Correlation of expression and activity of matrix metalloproteinase-9 and -2 in human gingival cells of periodontitis patients
김경아, Chung Soo-Bong, 황은영, Noh Seung-Hyun, 송권호, Kim Hanna-Hyun, 김철호, 박영국,
소속 상세정보
김경아 ( Kim Kyung-A ) - Kyung Hee University School of Dentistry Department of Orthodontics
( Chung Soo-Bong ) - Kyung Hee University School of Dentistry Department of Orthodontics
황은영 ( Hwang Eun-Young ) - Kyung Hee University School of Dentistry Department of Orthodontics
( Noh Seung-Hyun ) - Kyung Hee University School of Dentistry Department of Orthodontics
송권호 ( Song Kwon-Ho ) - Sungkyunkwan University Department of Biological Science
( Kim Hanna-Hyun ) - New York University College of Art and Sciences
김철호 ( Kim Cheorl-Ho ) - SungKyunKwan University Department of Biological Sciences
박영국 ( Park Young-Guk ) - Kyung Hee University School of Dentistry Department of Orthodontics
KMID : 0363020130430010024
Abstract
Purpose: Matrix metalloproteinases (MMPs) are capable of degrading extracellular matrix, and they are inducible enzymes depending on an inflammatory environment such as periodontitis and bacterial infection in periodontal tissue. Gingival inflammation has been postulated to be correlated with the production of MMP-2 and MMP-9. The objective of this study was to quantify the expression and activity of MMP-9 and -2, and to determine the correlation between activity and expression of these MMPs in human gingival tissues with periodontitis.
Methods: The gingival tissues of 13 patients were homogenized in 500 μL of phosphate buffered saline with a protease inhibitor cocktail. The expression and activity of MMP-2 and -9 were measured by enzyme-linked immunosorbent assay and Western blot analysis, and quantified by a densitometer. For the correlation line, statistical analysis was performed using the Systat software package.
Results: MMP-9 was highly expressed in all gingival tissue samples, whereas MMP-2 was underexpressed compared with MMP-9. MMP-9 activity increased together with the MMP-9 expression level, with a positive correlation (r=0.793, P=0.01). The correlation was not observed in MMP-2.
Conclusions: The expression of MMP-2 and -9 might contribute to periodontal physiological and pathological processes, and the degree of MMP-9 expression and activity are predictive indicators relevant to the progression of periodontitis.
키워드
Matrix metalloproteinase 2 ; Matrix metalloproteinase 9 ; Periodontitis
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